3/28 soil microbes lab report- discussion

Our hypothesis was that by isolating bacteria species JL and species SA from our sample auger dish, both isolated bacteria will produce an antibiotic that will inhibit the growth of the target bacteria, bacillus subtilis. Our prediction was that the purple bacteria will have a larger zone of inhibition compared to the white; however both will produce and antibiotic that will inhibit and kill the target bacteria. The data collected from this experiment was not consistent with our prediction because neither the purple bacteria nor the white bacteria produced a zone of inhibition. Our hypothesis was not supported by our data because the bacteria that was isolated did not produce antibodies that inhibited the growth of the target bacteria, bacillus subtilis. A logical explanation for why the isolated bacterium JL and SA did not inhibit the growth of the bacillus subtilis is that the target bacteria has developed immunity to the plug bacteria’s antibodies. A second explanation could be that the plugs do not produce antibodies at all. A third explanation could be the antibodies do not work against that specific target bacteria. Due to the possible explanations above, our hypothesis should not be entirely rejected because it could have been proven under different circumstances. If the target bacteria was a different type of bacteria instead of bacillus subtilis, the isolated bacterium JL and SA could have produced a zone of inhibition on that different type of bacteria and inhibited its growth. A logical modification of this experiment to address the issue that the antibodies do not work against bacillus subtilis would be to use a different target bacteria in the experiment to see if JL and SA produces antibodies that would effectively inhibit the growth of that different type of bacteria. In conclusion, through this experiment we learned that bacterium JL and SA do not produce antibodies to inhibit the growth of bacillus subtilis.

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